Multiwavelength anomalous diffraction (MAD)

From Online Dictionary of Crystallography

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Diffraction anomale multilongueurs d'ondes (Fr). Diffrazione anomala a più lunghezze d'onda (It)


An approach to solving the phase problem in protein structure determination by comparing structure factors collected at different wavelengths, usually from a tunable X-ray synchrotron source, including the absorption edge of a heavy-atom scatterer. Also known as multiple-wavelength anomalous diffraction or multiwavelength anomalous dispersion.


This technique was introduced by W. Hendrickson (Hendrickson, W. A., 1991, Determination of macromolecular structures from anomalous diffraction of synchrotron radiation. Science, 254, 51–58.)

See also

MAD and MIR. J. L. Smith, W. A. Hendrickson, T. C. Terwilliger and J. Berendzen. International Tables for Crystallography (2006). Vol. F, ch. 14.2, pp. 299-309 doi:10.1107/97809553602060000686